urc mondor

Unité de Recherche Clinique Henri Mondor

Maturation and persistence of the anti-SARS-CoV-2 memory B cell response.

Sokal A, Chappert P, Barba-Spaeth G, Roeser A, Fourati S, Azzaoui I, Vandenberghe A, Fernandez I, Meola A, Bouvier-Alias M, Crickx E, Beldi-Ferchiou A, Hue S, Languille L, Michel M, Baloul S, Noizat-Pirenne F, Luka M, Mégret J, Ménager M, Pawlotsky J-M, Fillatreau S, Rey FA, Weill J-C, Reynaud C-A, Mahévas M Cell. 2021;184(5):1201-1213.e14.

<p>Memory B cells play a fundamental role in host defenses against viruses, but to date, their role has been relatively unsettled in the context of SARS-CoV-2. We report here a longitudinal single-cell and repertoire profiling of the B cell response up to 6 months in mild and severe COVID-19 patients. Distinct SARS-CoV-2 spike-specific activated B cell clones fueled an early antibody-secreting cell burst as well as a durable synchronous germinal center response. While highly mutated memory B cells, including pre-existing cross-reactive seasonal Betacoronavirus-specific clones, were recruited early in the response, neutralizing SARS-CoV-2 RBD-specific clones accumulated with time and largely contributed to the late, remarkably stable, memory B cell pool. Highlighting germinal center maturation, these cells displayed clear accumulation of somatic mutations in their variable region genes over time. Overall, these findings demonstrate that an antigen-driven activation persisted and matured up to 6 months after SARS-CoV-2 infection and may provide long-term protection.</p>

MeSH terms: Adult; B-Lymphocytes; COVID-19; Flow Cytometry; Germinal Center; Humans; Immunologic Memory; Lymphocyte Activation; Middle Aged; Severity of Illness Index; Single-Cell Analysis; Spike Glycoprotein, Coronavirus
DOI: 10.1016/j.cell.2021.01.050